![]() ![]() The use of CD200R1 agonists to blunt the inflammatory response in the knee are insufficient to prevent disease progression in the mouse DMM model of OA without anatomical restoration of the normal joint biomechanics. We did not find any attenuation of cartilage degeneration or synovitis in DMM mice with CD200Fc or CCS13 at 12 weeks post-surgery, nor stereological differences in the properties of subchondral bone. Histopathological assessment was used as the primary outcome measure to assess cartilage and synovial changes, while ♜T imaging was used to compare the changes to the subchondral bone between untreated and treated arthritic groups. Oligonucleotides were injected intra-articularly beginning one week after surgery, with a total of six injections administered prior to sacrifice at 12 weeks post-surgery. CCS13 was evaluated as a therapeutic treatment along with CD200Fc and a phosphate-buffered saline vehicle control. ![]() Ten week old male C57BL/6 mice were randomized and surgical destabilization of the medial meniscus (DMM) to induce knee arthritis or sham surgery as a control were performed. This study aimed to determine whether CD200R1 agonists, namely the protein therapeutic CD200Fc or the synthetic DNA aptamer CCS13, both known to act as anti-inflammatory agents, are able to delay the pathogenesis of injury-associated knee osteoarthritis in a murine model. Therapeutic targeting of inflammatory activity in the knee immediately post injury may alter the course of osteoarthritis development. Post-traumatic knee osteoarthritis is characterized by cartilage degeneration, subchondral bone remodeling, osteophyte formation, and synovial changes. 6Division of Orthopedic Surgery, Department of Surgery, University of Toronto, Toronto, ON, Canada.5Krembil Research Institute, University Health Network, Toronto, ON, Canada.4Division of Orthopaedics, Osteoarthritis Research Program, Schroeder Arthritis Institute, University Health Network, Toronto, ON, Canada.3Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada.2Sunnybrook Research Institute, Toronto, ON, Canada.1Institute of Biomedical Engineering, University of Toronto, Toronto, ON, Canada.Rockel 4,5, Adam Hopfgartner 2, Mohit Kapoor 4,5,6, Jean Gariépy 2,3, Cari Whyne 1,2,6 and Diane Nam 2,6* Kathak Vachhani 1,2, Aaron Prodeus 2,3, Sayaka Nakamura 4,5, Jason S. ![]()
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